Gene Mapping and Expression of Tomato Bushy Stunt Virus

Purification of Tomato Bushy Stunt Virus by Differential Centrifugation

On the rigidity of RNA in tomato bushy stunt virus.

The motional state of RNA in tomato bushy stunt rirlls, both in thr crystalline st,attx and in solution, has been invrstigat,ed IuGng 31P nuclear magnetic resonance methods. It, has been found that t’hr RNA is highly immobile in tho nativ Crus. and it is suggested that t,he lack of a high-resolution X-ray diffraction patt,crn for either the RNB or the N-terminal repions of the pro&n coat molcrn...

Physicochemical Studies on Tomato Bushy Stunt Virus Variants

Tomato bushy stunt virus particles have previously been physically and chemically well characterized. Different strains, however, are known to have different host plants and symptoms, and they can be distinguished serologically. In an attempt to correlate this behavior with physicochemical properties, we have performed a comparative study on isolates of four of the main variants of this virus (...

Tomato bushy stunt virus (TBSV) infecting Lycopersicon esculentum.

Tomato bushy stunt virus (TBSV) was detected in tomato crop (Lycopersicon esculentum) in Egypt with characteristic mosaic leaf deformation, stunting, and bushy growth symptoms. TBSV infection was confirmed serologically by ELISA and calculated incidence was 25.5%. Basic physicochemical properties of a purified TBSV Egh isolate were identical to known properties of tombusviruses of isometric 30-...

Structure of Tomato Bushy Stunt Virus V.t Coat Protein Sequence Determination and its Structural Implications

CVe report the chemically determined sequence of most of the polypeptide chain of the coat protein of tomato bushy stunt virus. Peptide locations have been determined by comparison with the high-resolution electron density map from X-ray crystallographic analysis as well as by conventional chemical overlaps. Three small gaps remain in the 387-residue sequence. Positively charged sidechains are ...